Monday, December 25, 2017

Analytical techniques in genomics and proteomics + Bioinformatics

Analytical techniques in genomics and proteomics + Bioinformatics Eligibility: Undergraduate/post graduate students of Biology disciplines (Botany, Zoology, Biotechnology, Microbiology, Bio analytics, Biochemistry) Duration: 15 Days (5-6 hrs./day) 1. Plasmid DNA (vector) isolation from bacterial culture • Agarose gel electrophoresis • RFLP • Agarose gel electrophoresis 2. Theory on PCR and Primer design for PCR • Gene amplification by Polymerase chain reaction • Agarose gel electrophoresis to check gene amplification • Competent cell preparation, transformation and plating 3. Analysis of protein profile by SDS-PAGE • Casting a polyacryalamide gel and sample preparation and gel loading • Electrophoresis and staining the gel with Coomasie Blue/Silver for visualization of proteins Bioinformatics:  Nucleotide database (Genbank, EMBL, DDBJ)  Sequence retrieval systems (Entrez & SRS)  Database Search methods & tools,  PAM & BLOSUM Matrices, Algorithms- Dot Matrix, Smith waterman, Needleman-WUNSCH algorithm, BLAST, FASTA  Similarity, Identity Homology, Global Alignment, Fees: 7080 /- including GST

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